Faire le pH d'une solution de Tris

How to pH a solution

All solutions with defined pHs should be checked using the pH meter. pH paper is not accurate enough! The pH should be accurate to within 0.1 pH units.

If you are not sure how much acid or base you will need, make up the solution in a volume smaller than the final volume (e.g. 800 ml for 1 liter), adjust the pH and then bring to the final volume.

To adjust pH, use concentrated HCl (strong acid, kept in the Acids cabinet) or 10 N NaOH (strong base). You can also use dilute HCl (1 M) or 1 N NaOH for fine adjustment of pH. Don’t use NaOH pellets unless specified.

Adjust pH while the solution is being stirred in a beaker. Add the acid or base in small increments and check pH after each increment so as not to overshoot.

Concentrated HCl is a 37% solution (~11 M). To make 1 M HCl, dilute 86.5 ml of conc. HCl in 1 liter dH₂O.