mercredi 3 septembre 2008

Préparation de bleu de laemmli 6X SDS-PAGE Sample buffer

Recipe to prepare 10 ml:

- 1.2gr SDS (sodium dodecyl sulfate)
- 6mg bromophenol blue
- 4.7ml glycerol
- 1.2ml Tris 0.5M pH6.8
- 2.1ml distilled water warm it a little bit and shake it till everything is dissolved. It takes time.
- add 0.93gr DTT or bMe

Wait until it is completely dissolved.

Aliquote and keep frozen at-20ºC.

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