PCR touchdown manuelle

Programme PCR n°50
Stage 1
95°C X 2min X 1 cycle

Stage 2
92°C X 20"
64°C X 30" X 4 cycles
70°C X 30-60" (ou 1kb/min)

Stage 3
92°C X 20"
61°C X 30" X 4 cycles
70°C X (ou 1kb/min)

Stage 4
92°C X 20"
58°C X 30" X 4 cycles
70°C X (ou 1kb/min)

Stage 5
92°C X 20"
55°C X 30" X 25-35 cycles
70°C X (ou 1kb/min)

Stage 6
70°C X 5min X 1 cycle